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In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
|Assay Target Class||Phosphoprotein|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Quantity in a Package Amount||50000.0 Units|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System, Cardiovascular, Metabolic|
|Unit Size||50,000 Assay Points|
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