PerkinElmer
check quantity

HVEM (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for human herpesvirus entry mediator (HVEM) enables the quantitative determination of human HVEM (also known as TNFRSF14, CD270) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
AL3054HV
100 Assay Points
893.00 USD
 
more
AL3054C
500 Assay Points
2144.00 USD
 
more
AL3054F
5,000 Assay Points
17200.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor (TNFR) superfamily 14 and CD270, is a cell surface membrane protein that regulates T-cell immune responses by activating both inhibitory and inflammatory signaling pathways. HVEM is widely expressed on the plasma membranes of hematopoietic lineage cells, such as natural killer cells, monocytes, dendritic cells, neutrophils, T-cells and B cells. This membrane protein functions as a receptor and or ligand for BTLA, CD160, LIGHT and lymphotoxcin-alpha. Although little is known of the correlation of HVEM and cancer, it has been proven that elevated levels of HVEM have been associated in patients with autoimmune and allergic diseases such as atopic dermatitis, rheumatoid arthritis, and allergic asthma. This kit is designated to detect and quantify the levels of HVEM in cell culture supernatant and serum.

Specifications

Assay Target HVEM
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 5,000 Assay Points
Resources, Events & More
  • All

Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB

Application Note

Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA Technology

One approach to immunotherapy is the modulation of immune checkpoints that are critical in regulating the degree and duration of immune system responses and preventing autoimmunity.

In this application note, you will learn:

  • Why AlphaLISA® technology is an effective assay for the detection and quantification of biomarkers such as CD28 and CTLA-4
  • How to run an AlphaLISA assay to detect and quantify immuno-oncology biomarkers
  • Benefits of using Alpha technology for biomarker quantification assays
  • An example of the data and analysis you can generate for biomarker assays

PDF 1 MB

Brochure

Flyer

Manual

AlphaLISA human HVEM Detection Kit

This manual describes how to run an AlphaLISA human HVEM detection assay.

PDF 500 KB