AequoScreen Parental cell stably expressing the mitochondrially targeted apoaequorin gene. GPCRs of your choice can be transiently or stably transfected into these cell lines, for luminescence calcium assays.
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This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
Frozen Cells validated for calcium testing (HEK293 Parental cells). We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.
| Assay Target Type | Cell line |
|---|---|
| Assay Validated | Calcium Luminescence |
| Host Cell | HEK293 |
| Product Brand Name | AequoZen |
| Quantity in a Package Amount | 17.0 Million Cells |
| Second Messenger Release | Calcium flux |
| Shipping Condition | Dry Ice |
| Unit Size | 1 vial |
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.
