PerkinElmer
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Adenosine A2B AequoScreen; cell line

Stable, recombinant AequoScreen® cell line expressing aequorin and the Adenosine A2B receptor, human recombinant in HEK293 host cells.

Part Number ES-013-A
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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.

Overview

PerkinElmer’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.

The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z′, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

PerkinElmer’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.

Specifications

Assay Target Class GPCR
Assay Target Type Cell line
Assay Validated Calcium Luminescence
G-Alpha Coupling Protein Ga16
G-Alpha Natural Receptor Gs
Host Cell HEK293
Product Brand Name AequoScreen
Receptor Type Adenosine
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Special Ordering Information Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
Therapeutic Area Central Nervous System, Cardiovascular, Inflammation, Metabolic
Unit Size 5 million cells
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Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance

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Poster

Running an Aequorin Cell-Based Luminescent Assay on the FLIPR TETRA

We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR

PDF 270 KB