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Deoxythymidine 5'-Triphosphate, [Methyl-3H]-, Tetrasodium Salt, Specific Activity: 70-90 Ci (2.59-3.33 TBq)/mMole, 250 µCi (9.25 MBq)

dTTP, tritiated on the methyl group. This product is supplied in aqueous solution. dTTP that is tritiated on the methyl ground will incorporate labeled thymidine into DNA via DNA polymerase.

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Unit Size
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NET221A250UC
250 µCi
551.00 USD
 
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NET221A001MC
1 mCi
1046.00 USD
 
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NET221A005MC
5 mCi
2873.00 USD
 
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Overview

Deoxythymidine triphosphate (dTTP), labeled on the methyl group with 3H. Tritiated dTTP is commonly used to make probes for in situ hybridization studies via nick translation, or to measure nucleotide incorporation via reverse transcriptase or DNA polymerase activity.

References:

  1. Fonatsch, C., Gradl, G., Kolbus, U. et al. Hum Genet (1990) Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA, Human Genetics, 84:427.
  2. White, E.L., Parker, W.B., Macy, L.J., Shaddix, S.C., McCaleb, G., Secrist, J.A., Vince, R., Shannon, W.M. (1989) Comparison of the effect of Carbovir, AZT, and dideoxynucleoside triphosphates on the activity of human immunodeficiency virus reverse transcriptase and selected human polymerases, Biochemical and Biophysical Research Communications, 161(2):393-398.

Specifications

buffer Tricine
Detection Method Radiometric
Label Position Specifically Labeled
Molecular Weight 570.6
Nucleotide dTTP
Product Brand Name NEN Radiochemicals
Radioisotope H-3
Radionuclide 3H
Shipping Condition Dry Ice
Special Ordering Information This is a radioactive product - shipping address must have a license to receive radioactive materials.
Unit Size 250 µCi
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Radiometric Reagents Guide 2010-2011

For over 50 years PerkinElmer has been a leading supplier of radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments. Today is no different. We have always been committed to providing you products for all of your radiometric needs and we are still committed today.

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