PerkinElmer
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1/2 Area AlphaPlate-96, Light gray, 2 x 25 plates

96-well light gray 1/2 area microplate. Half-area plates utilize the same well spacing as a regular 96-well plate, but contain wells with half the diameter of a typical 96-well plate. This enables miniaturization to lower assay volumes while retaining ease-of-pipetting. Light gray plates can help minimize cross-talk while maintaining high signal in luminescent and Alpha assays.

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6002350
2 x 25 plates
257.00 USD
 
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8 x 25 plates
996.00 USD
 
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Overview

PerkinElmer's ½ AreaPlate offers same well depth at half the size to help you miniaturize your assay, designed to utilize cost reduction advantages without going to a 384-well format.

PerkinElmer is proud to have designed a light gray microplate which addresses unique challenges of sample screening. Our uniquely engineered 384 well and 1536 well light gray AlphaPlate microplates combine the best features of white microplates (excellent signal-to-background) and black microplates (decreased well-to-well crosstalk) for superior assay window and sensitivity in comparison to any other plate for luminescence assays. Our AlphaPlates are designed for a variety of technologies for luminescence based assays, but are specifically designed for use with PerkinElmer’s AlphaLISA and AlphaScreen technologies.

It’s important to choose the correct microplate prior to your screening project. By choosing the incorrect microplate, challenges such as missed data points, sample re-screening, missed project timelines and ultimately higher costs can be incurred.

Specifications

Automation Compatible Yes
Coating Treatment Untreated
Color Gray
Detection Method Luminescence, Alpha
Material Polystyrene
Product Brand Name ½ AreaPlate
Shipping Condition Ambient
Sterile No
Unit Size 2 x 25 plates
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Application Note

Rapid No Wash Assays for Characterizing a Mouse TIGIT/ PD-L1 Bispecific Antibody

Technical advancements in antibody engineering has brought about greater interest in more novel antibody therapeutic design and the emergence of new classes of antibody therapeutics called bispecific antibodies (bsAbs). The principle behind bispecific antibody design is to create an antibody / antibody fragment to two or more binding sites to help with the treatment of complex diseases.

As more bsAbs are produced as therapeutics, fast and accurate methods for functionally evaluating and characterizing the stability of these antibodies are necessary during both discovery and development stages, as well as during formulation and quality analysis.

In this application note, we demonstrate how AlphaLISA® assay technology with the EnVision® multimode plate reader can be used for bispecific antibody detection, through an example application to characterize the binding and specificity of a mouse bispecific antibody targeting mouse TIGIT and mouse PD-L1.

You will find out:

  • What bispecific antibodies are and how they differ from classical antibody formats
  • How to design quick and easy experiments to measure binding of the bispecific antibody to the target proteins
  • How a no-wash multiplex assay can show direct binding of each site to its respective target simultaneously in one well
  • How these assays could be used to evaluate the stability and functional reproducibility between batches of the bsAb preparations

PDF 1 MB