When it comes to the HPLC analysis of polar water-soluble compounds by reversed-phase chromatography, - things can get complicated and method simplification and reproducibility becomes even more important.
Polar water-soluble compounds are generally difficult to retain on hydrocarbon based (n-octyl or octadecyl) stationary phases and the separation of a mixture of water-soluble compounds on such types of columns can be challenging.
Many of us know or use methods that utilize ion-pairing mobile phase techniques to enable the retention and separation of polar analytes. However, many ion-pairing mobile phases are complicated and difficult to control.
PhD Matthew Przybyciel, R&D Leader at PerkinElmer presents how, even the most difficult analyses, can be simplified and demonstrates how the latest in column developments can enable easier and faster HPLC analysis of polar compounds for an end-to-end workflow solution that can improve your lab productivity.
In this exciting live webinar, you’ll learn about:
- Ways to tackle the most difficult HPLC polar compound analyses
- Which columns are best suited for polar compounds
- Simplifying methods and avoiding the use of ion-pairing mobile phases
- Food and pharma applications that require the retention and separation of polar compounds”