WEBINAR

Deriving Insights from 3D Cell Model Systems

Introduction

Part 4 of A Webinar Series: Towards Physiological Relevance - High Content Analysis with 3D Organoids and Spheroids

In this webinar, learn from experts about approaches to analyze and derive insights from 3D cell model systems using high-content analysis technology.

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In Situ Force Measurements Within 3D Microtissues

Dr. Eric M. Darling

Associate Professor of Medical Science, Orthopaedics, and Engineering Associate Professor of Medical Science, Orthopaedics, and Engineering

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Imaging and Analysis of 3D Cellular Models with PerkinElmer High-Content Imaging Systems

Dr. James Hutt

Field Applications Specialist in High Content Screening, PerkinElmer Inc.

Abstracts:

In Situ Force Measurements Within 3D Microtissues

Mechanical forces are an essential element to early tissue formation. However, few techniques exist that can quantify the mechanical microenvironment present within cell-dense neotissues and organoid structures. Dr. Darling will describe a versatile approach to measure microscale, cellular forces during mesenchymal condensation using specially tailored, hyper-compliant microparticles (HCMPs). Through monitoring of HCMP deformation over both space and time, measurements of the mechanical forces that cells exert, and have exerted on them, during tissue formation can be acquired.

You will learn:

  • How to use a fluorescence-based imaging system to measure microscale forces using discrete, in situ force sensors
  • How forces change based on time and location during mesenchymal condensation of adipose-derived stem cells
  • How force probe elasticity and cytoskeletal modulation can influence measured forces

Imaging and Analysis of 3D Cellular Models with PerkinElmer High-Content Imaging Systems

To better understand your 3D cell models and make insightful discoveries, full 3D volumetric analysis is possible in PerkinElmer’s Harmony® software. In a few mouse clicks you can easily render your data into 3D and visualize and interact with the samples. Harmony can perform 3D image segmentation enabling you to make quantitative measurements including: volume, morphology, intensity, texture, positions and distances in 3D. In this webinar Dr. Hutt will showcase the 3D viewing and 3D analysis of protrusions on cancer organoids.

Highlights include:

  • Overview of 3D models routinely assayed on PerkinElmer High Content instruments
  • Demonstrate Harmony software’s 3D capabilities
  • Showcase the analysis of protrusions of cancer organoids