This video, kindly provided by Immunocore, illustrates a high-content live-cell imaging assay which analyses ImmTAC®*- induced killing by T-cells (yellow) of antigen-positive cancer cells (blue).
In the assay, co-cultures of antigen-positive and antigen-negative cancer cells and T-cell effectors (effector to target ratio 1:1) in the presence of ImmTAC® were imaged every 10 min over 375 timepoints (62.5h in total) using the 40X water objective on the Opera Phenix high-content screening system.
- Antigen-positive cancer cells are stained with CellTracker™ Deep Red (blue)
- Antigen-negative cells are stained with CellTracker™ Red (white)
- Effector Pan T Cells are stained with CellTrace™ Violet (yellow).
- The cell death marker is NucView® 488 (green).
From the images, specificity, rate of killing and proliferation can be quantified using Harmony® image analysis software.
*For further details of Immunocore’s ImmTAC® technology, please visit www.immunocore.com/technology.