The key to cancer therapy utilizing microbes is the ability to track the spread and replication of the bacteria or viruses within the body over time. A number of strategies employing optical imaging for tracking reporter constructs in different models, from in vitro through to clinical samples have been developed. For bacterial work, novel reporter constructs for luminescence imaging of different genera in vitro and in vivo in mice have been generated. This has permitted 2D and 3D whole body imaging for a wide range species in different anatomical settings – in tumors, various deep organs, and even within the gastrointestinal tract of live animals. View this webinar to learn how Dr. Mark Tagney at Cork Cancer Research Center used IVIS® optical imaging to evaluate various luminescent and fluorescent reporters in vivo, ex vivo and in vitro in his research to investigate novel cancer therapies using microbes.