High-Throughput Phenotypic Profiling of ToxCast chemicals Using Cell Painting


Dr. Joshua A. Harrill
Cellular and Molecular Toxicologist, Center for Computational Toxicology and Exposure, US EPA

In this webcast, Dr. Harrill describes the use of an "in vitro pathology" assay known as Cell Painting for bioactivity profiling of environmental chemicals from the US EPA ToxCast collection. Information gleaned from this assay includes molecular point-of-departures (PODs) based on threshold concentrations for perturbation of cellular biology, and phenotypic profiles that can be used to identify chemicals that may be acting through similar mechanisms of action. Dr. Harrill demonstrates how this information can be used to inform next generation chemical safety assessments.


  • How the Cell Painting approach can be used to identify potency thresholds for chemical bioactivity
  • How Cell Painting can be used to inform mechanism-of-action prediction for uncharacterized or poorly characterized chemicals
  • Practical applications of high-throughput profiling data for chemical safety assessment

This abstract does not reflect USEPA policy.