Collection of Chromatography Methods for TCM & Alternative Medicines


Traditional Chinese medicine (TCM), herbal medicine and other alternative medicines have a rich history of healing and have been practiced for thousands of years. These treatments rely on analytical testing methods to meet pharmacopoeia standards such as Chinese Pharmacopoeia (ChP) and new, 2020 ChP standards. Due to the chemical diversity of TCM ingredients and finished products, chromatographic techniques are important to the characterization and identification needed to meet ChP standards.

Here, we’ve collected widely used TCM workflows utilizing the PerkinElmer UHPLC and SimplicityChrom software, LC/MS/MS and Simplicity 3Q software, PerkinElmer Epic columns, and a variety of chromatography consumables which enable the safety and quality ingredients in TCM products.

Select the TCM application note or brief you wish to download.

LC 300 TCM Resources HUB

Oleanolic Acid and Ursonic Acid in Eriobotrya Japonica Leaf

Eriobotrya japonica leaf is widely used as a medicinal material in treatments for a variety of chronic diseases. Key pharmacological components include triterpene acids, two of the marker compounds, oleanolic acid and ursonic acid are assayed in this method.

Paeoniflorin Analysis in Paeoniae Radix Alba

Paeoniflorin is an active component extracted from Paeoniae radix alba, which offers efficacy against gynecological disorders, pain, and hyperactivity of liver-yang, among other disorders. This method is for the quantification of paeoniflorin in herbal plants as per ChP specification.

chaenomelis fructus

Oleanlic Acid and Ursonic Acid Analysis of Chaenomelis Fructus

Chaenomeles, the fruit of the Chinese flowering quince, is utilized for its analgesic, anti-inflammatory, antispasmodic and astringent properties, and has also been shown to aid in digestion. Two components of the herbal plant are analyzed, oleanolic acid and ursonic acid.

Common Ginsenosides Found in Ginseng

Many natural products and herbal remedies are produced from the plant panax ginseng, which holds a wide range of medicinal properties such as antioxidant effects and promotion of immune function. Of the main active components, three ginsenosides are analyzed in this ChP method.

Ferulic Acid Analysis in Angelica Sinensis Radix by HPLC

Ferulic acid is an important and valuable phenolic acid whose content is used for assessing the quality of A. sinensis according to the Pharmacopoeia of China (ChP). This application brief outlines the analysis of identified market compound based on ChP.

QSight LC/MS/MS TCM Resources HUB

donkey hide gelatin

Workflow for Qualitative Identification of Donkey-Hide Gelatin

Donkey-hide gelatin has been used for centuries to optimize immune response, improve metabolomic balance and treat gynecological diseases. This brief outlines the total workflow solution for the qualitative identification of donkey-hide gelatin following ChP 2020.