Biocompatible Solid-Phase Microextraction and Laminar Flow Tandem Mass Spectrometry

A Convenient Solution for Quantitative Analysis of Biofluids


Featured Speaker:

Emanuela Gionfriddo, Ph.D.
Assistant Professor, The University of Toledo, OH

The ultra-trace determination of small organic molecules in complex biospecimens, such as plasma, requires sample preparation/extraction techniques able to pre-concentrate the analytes of interest and minimize matrix co-extractants that can potentially affect the accuracy of the analysis. Moreover, robust analytical instrumentation is critical to ensure adequate reproducibility and signal stability at trace levels. This webinar presents the development and validation of a new analytical protocol, based on bio-compatible Solid-Phase Microextraction (SPME) and LC-MS/MS to test the bioavailability, adsorption kinetics and metabolic interconversion of nicotine in rabbit plasma after application of smoke cessation formulations.

Dr. Emanuela Gionfriddo is an Assistant Professor of Analytical Chemistry at the Department of Chemistry & Biochemistry of The University of Toledo (OH, USA). Research work in Dr. Gionfriddo’s lab focuses on the development of advanced analytical separation tools for the analysis of complex biological & environmental matrices, with emphasis on alternative green sample preparation methodologies. She received her B.Sc. (2008) & M.Sc. (2010) in Chemistry & her Ph.D. in Analytical Chemistry (2013) at the University of Calabria (Italy). She joined Prof. Pawliszyn’s group at the University of Waterloo (Ontario, Canada) in 2014 as Post-Doctoral Fellow & manager of the Gas-Chromatography section of the Industrially Focused Analytical Research Laboratory (InFAReL), & within three years became a Research Associate. Dr. Emanuela Gionfriddo has currently authored 30 research articles, 8 reviews, a book chapter, & a patent on PTFE-based SPME coatings. Dr. Gionfriddo is one of the founding members of the Dr. Nina McClell& Laboratory for Water Chemistry & Environmental Analysis at the University of Toledo & she is appointed to the Ohio Attorney General Yost’s Environmental Council of Advisors.