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Technical Note

Using the VICTOR Nivo for Cell-Based HTRF Assays

Introduction

Cell-based assays are routinely employed in R&D to reveal compound induced effects in vitro. In this technical note, we describe the usage of the VICTOR® Nivo multimode plate reader for alpha-tubulin acetyl-K40 detection in a cellular assay based on HTRF® technology. Tubulin acetylation is widely studied due to its dysregulation in human neurological disorders. To demonstrate the HTRF assay principle on the VICTOR Nivo, we used mouse fibroblasts (NIH-3T3 cells).

The HTRF certified VICTOR Nivo optimally supports the application of HTRF kits. Learn how easy it is to use the cell-based alpha-tubulin acetyl-K40 HTRF assay kit on the instrument.