PerkinElmer

Application Note

Western Blot Validation of CRISPR-Cas9-induced Functional Knockout using Synthetic sgRNA Molecules

Introduction

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon Discovery’s Edit-R predesigned synthetic sgRNAs result in high efficacy knockout of target genes as validated by western blot using PerkinElmer’s Western Lightning ONE chemiluminescent substrates.