Application Note

Homogeneous Detection of Cytokines from PBMC Supernatants using AlphaLISA High Performance Assays


Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as well as the pathology of many diseases including cancer, autoimmune conditions, neurodegenerative disorders, and atherosclerosis. As such, cytokines are often used either directly or indirectly as targets of drug discovery for the development of new immunomodulatory therapies.

In this application note, we demonstrate how to easily detect and quantify three cytokines, interferon gamma (IFNγ), interleukin 2 (IL-2), and tumor necrosis factor alpha (TNFα) with AlphaLISA High Performance Detection Kits. This offers a homogenous, highly sensitive, and fast assay for furthering your cytokine research. Learn more in this detailed application note!