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Application Note

Tracking Inflammasome Activity via Measuring IL-1β levels with AlphaLISA and HTRF

Introduction

It has been suggested that inflammation promotes malignancy via proinflammatory cytokines, such as IL-1ß, which enhance immune suppression through the induction of myeloid suppressor cells (MSC), thereby counteracting immune surveillance and allowing the outgrowth and proliferation of malignant cells.

While there are multiple immunoassay options to choose from including ELISA, this application note demonstrates the utility of AlphaLISA® and HTRF® for the detection of IL-1ß in cell supernatant and serum samples.