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Application Note

Rapid Small-Scale Protein Purification and Characterization using the JANUS G3 BioTx Workstation

Introduction

Antibody-based therapies are one of the largest areas of therapeutics. The developmental stages require researchers to prepare, purify and analyze thousands of samples. Small-scale purification reduces the time needed to purify samples and accelerates the development of biotherapeutics. However, since small-scale purification increases throughput, protein characterization and analysis become the bottlenecks in the workflow. Methods such as ELISA, SDS-PAGE, capillary electrophoresis and HPLC require manual sample handling and/or analysis times of up to 30 minutes per sample. The need to analyze more samples creates the demand for a high-throughput analytical platform with superior precision, automation, and ease of use.

Purolite® partnered with PerkinElmer to show that, even when using different types of protein A, there would not be any significant difference in clearance performance under the process conditions typically used to purify full-length human antibodies. By using PerkinElmer technologies, Purolite® was able to save time and money while producing highly purified eluates that are straightforward to analyze.

For research use only. Not for use in diagnostic procedures.