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Application Note

Rapid Analysis of Gelatinous Traditional Chinese Medicine Using an Epic C18 UHPLC Column

Introduction

Gelatinous Traditional Chinese Medicines (TCMs), utilized to nourish yin, replenish blood, and prolong life, are made from animal skins, shells and horns, such as donkey-hide gelatin, tortoise-shell glue and deer-antler glue. Owing to the high market price and shortage of raw materials, adulteration from pig, cattle or similar non-toxic materials is common to substitute or replace the authentic materials. To combat this adulteration, Chinese Pharmacopeia (ChP) has developed qualitative identification methods using LC/MS/MS to test the quality of gelatinous TCM products through analyzing biomarker tryptic-digested peptides.

In this application brief, a quick method for the analysis of gelatinous TCMs, which can also be applied to other complex matrices such as peptides or polymers, utilizing a PerkinElmer QSight® LX50 UHPLC system coupled to a QSight 220 triple quadrupole mass spectrometer, with Simplicity 3Q software platform, a PerkinElmer Epic C18 UHPLC column, and PerkinElmer consumables is presented.