Application Note

How to Design a Column Screening Kit for Preparative SFC Separations


The utilization of supercritical fluid chromatography (SFC) provides a number of advantages to chromatographers. These include the “green” aspect of utilizing carbon dioxide as a mobile phase, the ease of isolation of purified components from preparative separations, and decreased analysis times because of the high diffusivity and low resistance to mass transfer encountered in SFC. The authors of this study demonstrate how to develop a column screening kit with particular attention being paid to the preparative SFC chromatography and its use for the purification/isolation of both chiral and achiral compounds.