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Application Brief

HPLC Analysis of Hydrocortisone in Accordance with the United States Pharmacopeia

Introduction

Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application brief describes the assay analysis of hydrocortisone in accordance with USP monographs using a PerkinElmer LC 300 liquid chromatography system with SimplicityChrom chromatography data system (CDS) and PerkinElmer Epic columns.