PerkinElmer

Application Brief

Tanshinone and Derivatives Analysis in Radix Salviae Miltorrhizae by HPLC

Introduction

Radix Salviae Miltorrhizae, called Danshen in Chinese Traditional Medicine (TCM), has been used to treat cardiovascular disease for more than a millennium. It is the root and rhizomes of the plant Salvia Miltiorrhiza Bunge of the Lamiaceae family. Pharmacological studies showed that nonpolar compounds such as tanshinone IIA, tanshinone I, and cryptotanshinone are active components with a variety of biological activities including antiischemic, antioxidant, and antitumor properties.

In this work, quantification of tanshinones in herbal plant based on the Chinese Pharmacopoeia method was performed using a PerkinElmer LC 300 HPLC system, an Epic C18 column, and SimplicityChrom software (Figure 2). A regulatory quality monitoring workflow for quantification of the multiple marker compounds was established.