Technical Note

A Comparison of Quasar C18 and Inertsil ODS-3 HPLC Columns


The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bondedsilica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. Simply swapping a C18 column from one manufacturer to another can result in differences in retention time, resolution and even selectivity. Differences can arise due to variations in hydrophobicity, silanol activity, packing quality, particle size distribution, and silica purity, to name a few.1

This technical note provides details of a comparative study between PerkinElmer Quasar™ C18 and GL Sciences Inertsil ODS-3 silica-based columns (150 x 4.6 mm, 5 µm), covering the following areas:

  • Relative Hydrophobicity
  • Efficiencies for a Neutral Compound
  • Evaluation of Silanol Activity
  • Application example – analysis of clotrimazole