Technical Note

Clone Columns-Harmony C18 and Waters Symmetry C18 Comparison


Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with compliant procedures, any unplanned downtime to investigate OOS instances can impact productivity. Our range of clone phases offer a cost-effective comparable alternative to many of the older leading brands, whilst ensuring consistency and stability in analysis. Improved lot-to-lot reproducibility is also achieved due to more stable production methods especially when compared to older brands.

The HarmonyTM C18 provides equivalent selectivity to the Waters Symmetry® C18 HPLC column and many chromatographers use Harmony when looking for a Symmetry equivalent. Harmony offers excellent column lifetime and reproducibility, and is engineered to the tightest specifications. It is also available in large scale bulk. Other Harmony phases are also available including C8 and C4.

This technical note provides examples of comparative studies between our Harmony C18 and the Waters Symmetry C18 phases for the following pharmaceutical drug applications:

  • Fluconazole
  • Betamethasone dipropionate and beclomethasone dipropionate
  • Acetaminophen, aspirin, caffeine and benzoic acid