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Technical Note

Time-Resolved Live-Cell Cytotoxicity Assay in 2D and 3D Using the Muvicyte Live-Cell Imaging System

Introduction

Cytotoxicity assays are often performed as endpoint assays, but a kinetic or time-resolved assay enables more precise assessment of cytotoxicity, especially if the kinetics of the compounds are unknown or varied, or if cells display different proliferation rates.

Assessment of cytotoxicity can also be affected by the type of cell model, e.g. 2D or 3D, or the use of fluorescent dyes which, over a long time-course, can have negative effects on metabolism and proliferation and hence on responses to the exogenous factors.

In this technical note, we describe label-free analysis of cell growth in monolayer and spheroid growth in 3D using brightfield images in order to determine cytotoxic effects, and compare the responses of three different cell lines to test compounds in both 2D and 3D.

Download our technical note to learn how you can:

  • Accurately quantify cytotoxicity in a variety of cell models in 2D and 3D
  • Measure individual responses to compounds in long term kinetic assays
  • Quantify 2D confluency and spheroid growth in brightfield images, avoiding the use of a fluorescent dye