Application Brief

HPLC Analysis of Ibuprofen in Water Using a Quasar SPP C18 Column


Pharmaceuticals are continuously entering our waterways via human excretion of incompletely absorbed medication and improper disposal of unused drugs via drains and toilets. A report found that the six most consistent highest reported concentrations in finished drinking water for active pharmaceutical ingredients (APIs) were from ibuprofen, triclosan, carbamazepine, phenazone, clofibric acid, and acetaminophen.

Ibuprofen (Figure 1) is a widely used analgesic and antipyretic for adults and children and since it’s one of the most reported pharmaceutical in finished drinking waste, it is important to determine the amount removed by different water treatment processes. HPLC is an essential technique to determine the quantity of ibuprofen in water and help optimise a process for its removal. This application brief describes the use of a Quasar superficially porous particles (SPP) C18 column for the analysis of ibuprofen in water.