PerkinElmer
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Application Note

Quantification of Proteins by UV-Vis Absorbance Using VICTOR Nivo with Microvolume Plates

Introduction

Knowing the protein concentration is commonly required in studies of protein biochemistry and molecular biology. A direct method of measuring protein concentration is to determine the absorbance spectrum at 280 nm. With this UV based approach, it enables scientists to rapidly determine the concentration of protein with a procedure that is simple and nondestructive, does not need special reagents or standard curves, and consumes very little sample.

In this study, learn why the VICTOR® Nivo multimode plate reader is an ideal instrument for UV/Vis analysis of proteins with a µDrop Plate. This combination provides robust and fast read-out of a single sample or up to 16 reads at once.