PerkinElmer
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Application Note

Rapid HPLC Analysis of Retinol Using a Quasar C18 Column

Introduction

Retinol, one of the main forms of vitamin A, is regularly used in the treatment and protection of skin. By inducing the biosynthesis of collagen and promoting skin cell production, retinol helps to reduce the signs of early UV-induced skin aging. Retinol is also an anti-oxidant, allowing it to neutralize free radicals that may damage healthy skin cells. HPLC with UV detection is commonly used to assess and quantify retinol in a variety of samples.3 This application brief describes the use of a Quasar C18 column for the rapid analysis of retinol.