PerkinElmer
Imaging Bile Canaliculi in 3D Liver Microtissues

Application Note

Imaging Bile Canaliculi in 3D Liver Microtissues

Imaging Bile Canaliculi in 3D Liver Microtissues

Introduction

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.