Current trends for the analysis of the cannabinoid content in cannabis flower extracts/concentrates and commercially available fortified foods depend on liquid chromatography for potency testing and/or ensuring the label-claim accuracy in product content descriptions. This application note describes a fast and robust chromatographic method for the separation and quantitation of 16 cannabinoids commonly analyzed in cannabis-centric labs, using the PerkinElmer Flexar HPLC system with a PDA detector. To further validate the performance of this method for the industry, The Emerald Test Proficiency Test (PT) for Potency was conducted. The Emerald Test™ is an Inter-Laboratory Comparison and Proficiency Test (ILC/PT) program for cannabis testing labs. The results from the PT inter-laboratory samples passed; therefore, the method meets inter-laboratory reproducibility and accuracy.