PerkinElmer
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Application Note

Effects of 5FU and Sorafenib on Proliferation and Biomarker Expression in a Colorectal Cancer Model Using AlphaLISA and EnSight Solutions

Introduction

A variety of chemotherapeutic drugs with different modes of action have been developed and tested as potential therapies for colorectal cancer. Characterizing the effects of potential drugs with different modes of action is a key part of the process.

In this application note you will learn:

  • How to rapidly measure multiple biomarkers in both cell culture supernatant and lysates from the same wells of a microplate to examine complex protein expression profiles from a colorectal cancer cellular model
  • Benefits of using AlphaLISA® technology for characterizing the effects of potential drugs with different modes of action on a cell culture model of human colorectal cancer
  • How to measure the effects of drug treatments, such as 5FU and Sorafenib, on cellular proliferation by automated well-imaging and cell counting using the EnSight® multimode plate reader
  • Examples of the data and analysis you can generate for such biomarker and proliferation assays