Various cytokines are secreted during an active immune response that can have modulatory effects on target cell populations, including interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNFa) and several interleukins.
In this application note, you will learn how we investigated:
- The effects of secreted factors on a triple-negative breast cancer cell line by treatment with conditioned media from activated PBMCs
- Whether the modulatory effects of cytokines secreted by infiltrating immune is different when studied in 3D cell cultures
- How to detect and quantify multiple immune-modulated proteins from the same wells in complex cell models grown in 3D spheroid microplates and traditional 2D cell culture using AlphaLISA® technology and EnVision® multimode plate reader in a workflow with ATPlite™ 1step and Opera® Phenix high-content screening system