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Application Note

UHPLC-PDA Analysis of Curcuma Using a Quasar SPP Column

Introduction

Turmeric is the powdered dry rhizome of the plant Curcuma longa L., and is used as a coloring agent to give a yellow-orange color to food dishes.

It has been found to be a rich source of phenolic compounds, namely, three curcuminoids: curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Research to date suggests that turmeric, in addition to having an immunomodulatory role, is also of use in preventing oxidative stress that can lead to inflammation, cancer, and arthritis. This application note describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of the three curcuminoids in commercially available turmeric spices and roots by UHPLC, using photodiode array (PDA) detection.