PerkinElmer
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Technical Note

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Introduction

Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. However, setting up live cell experiments can be challenging, as temperature, CO2 and evaporation need to be controlled to ensure optimal cell growth conditions. In this technical note, we demonstrate:

  • Successful five day live cell imaging on Operetta CLS and Opera Phenix high-content systems
  • Avoidance of phototoxicity with gentle digital phase contrast imaging
  • Analysis of cell growth and morphology on a single cell level without any fluorescence staining