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Application Note

FcRn Binding Assays using AlphaLISA® and HTRF® Technologies

Introduction

This application note shows you how to determine the relative affinity of different therapeutic antibodies to FcRn, and how it can be used to compare biosimilars. It contains experiments and data that investigate the specificity of the assays for binding the Fc portion of an antibody, and the binding performance of different subtypes. It features:

  • Assay principle and workflow
  • Consistent results (data and graphs)
  • Informative materials and methods, including references