PerkinElmer
Brief PMS299 D

Application Note

Water Analysis using LAMBDA Spectrophotometer: Nitrate Nitrogen (NO3-N), Brucine Method

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Introduction

When a water sample containing nitrate ions is treated with brucine in sulfuric acid condition, a yellow compound is created. The quantity of nitrate nitrogen can be determined by measuring the absorbance of the yellow compound at 410 nm. In this application note, the quantitative analysis of Nitrate nitrogen (NO3-N) was performed by Brucine method. Data was rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab™ Software.