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Technical Note

Opera Phenix High-Content Screening System: Improved 3D Imaging

Introduction

Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’ which can lead to blurred images in conventional spinning disk systems. The confocal scanning unit of the Opera Phenix High-Content Screening System provides an increased distance between pinholes to reduce the pinhole crosstalk, leading to improved 3D image quality. Learn more in our Technical Note.