Due to it’s serious impact on world health, society and the economy, understanding the extent of the COVID-19 pandemic is an ongoing key challenge for public health officials, even in consideration of the vaccine roll out.
Wastewater monitoring, also known as wastewater-based epidemiology (WBE), continues to play a crucial part for early identification of SARS-CoV-2 infection rates and hot spots and the surveillance of COVID-19. The demand for highly sensitive, mass-scale, cost effective and non-invasive solutions for the surveillance of the infection continues to be great.
In this Application Brief, global genomics sequencing laboratory, Dante Labs, has demonstrated how the identification of the SARS-CoV-2 virus within wastewater samples is achievable through both manual and automated workflows. The automated workflow that Dante Labs utilized includes RNA isolation sample prep with the use of the chemagic™ Viral DNA/RNA kit special H96 with the chemagic™ 360 instrument for RNA extraction, the JANUS® G3 PCR Workstation for automated SARS-CoV-2 PCR assay setup and SARS-COV-2 RT-PCR kit from PerkinElmer.