PerkinElmer
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Application Brief

Detection of SARS-CoV-2 in Wastewater

Introduction

Due to it’s serious impact on world health, society and the economy, understanding the extent of the COVID-19 pandemic is an ongoing key challenge for public health officials, even in consideration of the vaccine roll out.

Wastewater monitoring, also known as wastewater-based epidemiology (WBE), continues to play a crucial part for early identification of SARS-CoV-2 infection rates and hot spots and the surveillance of COVID-19. The demand for highly sensitive, mass-scale, cost effective and non-invasive solutions for the surveillance of the infection continues to be great.

In this Application Brief, global genomics sequencing laboratory, Dante Labs, has demonstrated how the identification of the SARS-CoV-2 virus within wastewater samples is achievable through both manual and automated workflows. The automated workflow that Dante Labs utilized includes RNA isolation sample prep with the use of the chemagic Viral DNA/RNA kit special H96 with the chemagic 360 instrument for RNA extraction, the JANUS® G3 PCR Workstation for automated SARS-CoV-2 PCR assay setup and SARS-COV-2 RT-PCR kit from PerkinElmer.