Technical Note

3D Volumetric and Zonal Analysis of Solid Spheroids

3D volumetric and zonal analysis of solid spheroids


Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

In this technical note we describe how to image and analyze solid spheroids in 3D using the Opera Phenixand OperettaCLS high-content screening systems and Harmony® 4.8 imaging and analysis software.

We address:

  • How to reduce image acquisition time and data volume
  • How to analyse fully and partially imaged spheroids in 3D
  • How to define zones and quantify spatial differences within spheroids