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JoVE Online Video Presentations

Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed indexed journal devoted to the publication of biological, medical, chemical and physical research in a video format.  The video-articles listed below use PerkinElmer in vivo imaging systems and/or imaging reagents.  Video-articles are either open access, in which you can view the full video or a subscription is required to view the video in its entirity.

Open Access Videos

Utilizing F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Milica Momcilovic1, Sean T. Bailey2, Jason T. Lee3, Charles Zamilpa3, Anthony Jones3, Gihad Abdelhady1, James Mansfield4, Kevin P. Francis5, David B. Shackelford1
1Division of Pulmonary and Critical Care Medicine, University of California Los Angeles David Geffen School of Medicine, 2University of North Carolina at Chapel Hill, 3Department of Molecular and Medical Pharmacology, University of California Los Angeles, 4Andor Technology, 5Division of Orthopaedic Surgery, University of California Los Angeles David Geffen School of Medicine



JoVE video 53603

In vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability using MicroCT Imaging

Elza van Deel1,2, Yanto Ridwan1, J. Nicole van Vliet1, Sasha Belenkov3, Jeroen Essers1,4,5
1Department of Genetics, Erasmus MC, Rotterdam, 2Department of Experimental Cardiology, Erasmus MC, Rotterdam, 3PerkinElmer, Inc., 4Department of Vascular Surgery, Erasmus MC, Rotterdam, 5Department of Radiation Oncology, Erasmus MC, Rotterdam



JoVE video 51612

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Nicholas M. Bernthal1, Brad N. Taylor2, Jeffrey A. Meganck2, Yu Wang3, Jonathan H. Shahbazian3, Jared A. Niska1, Kevin P. Francis2, Lloyd S. Miller3,4
1Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), 2PerkinElmer, 3Department of Dermatology, Johns Hopkins University School of Medicine, 4Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine

Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone.

PRODUCTS: IVIS Spectrum, Quantum FX, Living Image software, Xen29 bioluminescent Staphylococcus aureus strain


JoVE video 51790

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Xueli Zhang1,2, Chaincy Kuo3, Anna Moore1, Chongzhao Ran1
1Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, 2Center for Drug Discovery, School of Pharmacy, China Pharmaceutical University, 3Perkin Elmer

In this video report, we show the application of Cerenkov Luminescence Imaging (CLI) for interscapular brown adipose tissue in mice under activated and depressed conditions.



JoVE video 50450

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

James William Collins1, Jeffrey A Meganck2, Chaincy Kuo2, Kevin P Francis2, Gad Frankel1
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology, Imperial College London, 2Preclinical Imaging, PerkinElmer

Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.

PRODUCTS: IVIS SpectrumCT, XGI-8 gas anesthesia system, Living Image 4.3.1 software


JoVE video 4429

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

Allison Poussard*, Michael Patterson*, Katherine Taylor, Alexey Seregin, Jeanon Smith, Jennifer Smith, Milagros Salazar, Slobodan Paessler
Experimental Pathology, University of Texas Medical Branch

Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study.

PRODUCTS: IVIS Spectrum, XGI-8 gas anesthesia system, Living Image 4.0 software


JoVE video 3246

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Madonna M. McManus, Raymond J. Grill
Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston

A longitudinal examination of bone loss in the femurs and tibiae of adult mice was performed following spinal cord injury using sequential low-dose X-ray scans. Tibia bone loss was detected throughout the study, while bone loss in the femur was not detected until 40 days post injury.  



JoVE video 52462

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Peter Bannas*1, Alexander Lenz*1,2, Valentin Kunick1,2, William Fumey1,2, Björn Rissiek2, Joanna Schmid1,2, Friedrich Haag2, Axel Leingärtner3, Martin Trepel4, Gerhard Adam1, Friedrich Koch-Nolte2
1Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, 2Institute of Immunology, University Medical Center, Hamburg, 3University Cancer Center Hamburg, University Medical Center, Hamburg, 4Department of Oncology and Hematology, University Medical Center, Hamburg

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.




Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models




Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

PRODUCTS:  IVIS Spectrum, Luciferin



In vivo Dual Substrate Bioluminescent Imaging

PRODUCTS: IVIS 200, RediJect Coelenterazine, D-Luciferin



Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems




Ex Vivo Infection of Live Tissue with Oncolytic Viruses




Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice




Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

PRODUCTS:  IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware)



In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

PRODUCTS: IVIS Spectrum, D-Ludiferin



Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging




In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum




Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging




In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum




Use of Human Perivascular Stem Cells for Bone Regeneration

PRODUCTS:  IVIS Lumina II, Living Image



Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

PRODUCTS:  IVIS Imaging System, D-Luciferin



A JoVE Subscription is Required To View These Videos in Their Entirity

JoVE video 52771

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Lukasz J. Ochyl1,2, James J Moon1,2,3
1Department of Pharmaceutical Sciences, University of Michigan, 2Biointerfaces Institute, University of Michigan, 3Department of Biomedical Engineering, University of Michigan

We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.



JoVE video 51174

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Malek Saleh1, Mohammed R. Abdullah1, Christian Schulz1, Thomas Kohler1, Thomas Pribyl1, Inga Jensch1, Sven Hammerschmidt1
1Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald

Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

PRODUCTS: IVIS Spectrum, Living Image 4.1 Software, XGI-8 Gas Anesthesia System


JoVE video 50690

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

Jen-Chieh Tseng1, Andrew L. Kung2
1Lurie Family Imaging Center, Dana-Farber Cancer Institute, Harvard Medical School, 2Division of Pediatric Hematology/Oncology/Stem Cell Transplantation, Columbia University Medical Center

We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.

PRODUCTS: IVIS Spectrum, Living Image 4.2 Software


JoVE video 4341

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

Yingding Xu, Hongguang Liu, Edwin Chang, Han Jiang, Zhen Cheng
Department of Radiology and Bio-X Program Canary Cancer at Stanford for Cancer Early Detection, Stanford University

Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening.




Bioluminescent Bacterial Imaging In Vivo




Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme




Models of Bone Metastasis

PRODUCTS: IVIS Imaging System



Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic




Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model




Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

PRODUCTS: D-Luciferin



An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

PRODUCTS: IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware)



A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

PRODUCTS: IVIS Spectrum, D-Luciferin



In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model




Using Luciferase to Image Bacterial Infections in Mice

PRODUCTS: IVIS Imaging System



An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses




In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

PRODUCTS: IVIS 200, D-Luciferin



Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

PRODUCTS: IVIS 200, D-Luciferin



Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia




In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

PRODUCTS: IVIS Imaging System



DNA Vector-based RNA Interference to Study Gene Function in Cancer

PRODUCTS: IVIS Imaging System



Murine Bioluminescent Hepatic Tumour Model

PRODUCTS: IVIS Imaging System



Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

PRODUCTS: IVIS Imaging System