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LANCE Citations

On this page we provide selected citations for each application.

LANCE cAMP assays


Journal articles

  1. Ben-Shlomo, A. et al. Constitutive Somatostatin Receptor Activity Determines Tonic Pituitary Cell Response. Mol Endocrinol 23, 337-348 (2009). Link
  2. Chen, C., McLachlan, S.M. & Rapoport, B. Identification of Key Amino Acid Residues in a Thyrotropin Receptor Monoclonal Antibody Epitope Provides Insight into Its Inverse Agonist and Antagonist Properties. Endocrinology 149, 3427-3434 (2008). Link
  3. Doucette, C. et al. Kappa Opioid Receptor Screen with the Tango Beta-Arrestin Recruitment Technology and Characterization of Hits with Second-Messenger Assays. J Biomol Screen 14, 381-394 (2009). Link
  4. Hamilton, B.S. & Doods, H.N. Identification of potent agonists acting at an endogenous atypical Beta3-adrenoceptor state that modulate lipolysis in rodent fat cells. Eur J Pharmacol 580, 55-62 (2008). Link
  5. Hendriks-Balk, M.C. et al. S1P Receptor Signalling and RGS Proteins; Expression and Function in Vascular Smooth Muscle Cells and Transfected CHO Cells. Eur J Pharmacol 600, 1-9 (2008). Link
  6. Knight, J.A., Smith, C., Toohey, N., Klein, M.T. & Teitler, M. Pharmacological Analysis of the Novel, Rapid, and Potent Inactivation of the Human 5-Hydroxytryptamine7 Receptor by Risperidone, 9-OH-Risperidone, and Other Inactivating Antagonists. Mol Pharmacol 75, 374-380 (2009). Link
  7. Mizutori, Y., Chen, C., Latrofa, F., McLachlan, S.M. & Rapoport, B. Evidence that Shed Thyrotropin Receptor A Subunits Drive Affinity Maturation of Autoantibodies Causing Graves' Disease. J Clin Endocrinol Metab 94, 927-935 (2009). Link
  8. Xia, M. et al. Identification of Compounds that Potentiate CREB Signaling as Possible Enhancers of Long-Term Memory. P Natl Acad Sci USA 106, 2412-2417 (2009). Link


LANCE Ultra cAMP journal articles

  1. Townsend, E. A., Sathish, V., Thompson, M. A., Pabelick, C. M. & Prakash, Y. S. Estrogen effects on human airway smooth muscle involve cAMP and protein kinase A. Am. J. Physiol. Lung Cell Mol. Physiol. 303, L923–928 (2012).
  2. Blättermann, S. et al. A biased ligand for OXE-R uncouples Gα and Gβγ signaling within a heterotrimer. Nat. Chem. Biol. 8, 631–638 (2012).
  3. Oddi, S. et al. Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling. Br. J. Pharmacol. 165, 2635–2651 (2012).
  4. Li, Y. et al. Identification of two functionally distinct endosomal recycling pathways for dopamine D₂ receptor. J. Neurosci. 32, 7178–7190 (2012).
  5. Nikas, S. P. et al. Novel 1’,1’-chain substituted hexahydrocannabinols: 9β-hydroxy-3-(1-hexyl-cyclobut-1-yl)-hexahydrocannabinol (AM2389) a highly potent cannabinoid receptor 1 (CB1) agonist. J. Med. Chem. 53, 6996–7010 (2010).
  6. Schröder, R. et al. Applying label-free dynamic mass redistribution technology to frame signaling of G protein-coupled receptors noninvasively in living cells. Nat Protoc 6, 1748–1760 (2011).

PerkinElmer technical publications
  1. Hurt, S. et al. Automation of a GPCR Cell-based Functional Assay on the Cellular Workstation System using the LANCE cAMP Technology. PerkinElmer Poster. Link
  2. Hurt, S., Le, H., Kaur, N. & Rayl, B. Use of the JANUS Cellular Workstation for the Automation of GPCR Cell-Based Functional Assays Using LANCE cAMP Technology. PerkinElmer Poster. Link
  3. Kasila, P. and Harney, H. LANCE cAMP - Increased Sensitivity, Stability, and Overall Assay Performance with Gαs and Gαi coupled receptors in a miniaturized 1536-well format. PerkinElmer Poster. Link
  4. Kasila, P. and Xie, H. LANCE cAMP - A uHTS cAMP detection assay for use with membranes. PerkinElmer Poster. Link
  5. Kasila, P., Warner, G., Stuchly, C., Evans, K.L. & Fletcher, P. A Phosphodiesterase Assay using LANCE Technology: Simple, Flexible and Sensitive. PerkinElmer Poster. Link


LANCE biomolecular interaction assays


Journal articles

  1. Mitro, N., Godio, C. & Crestani, M. Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators. Methods Mol. Biol. 952, 219–227 (2013).
  2. Dams, G. et al. A Time-Resolved Fluorescence Assay to Identify Small-Molecule Inhibitors of HIV-1 Fusion. J Biomol Screen 12, 865-874 (2007). Link
  3. Lindqvist, L. et al. Selective Pharmacological Targeting of a DEAD Box RNA Helicase. PLoS ONE 3, e1583 (2008). Link
  4. Mehle, A. et al. Identification of an APOBEC3G Binding Site in Human Immunodeficiency Virus Type 1 Vif and Inhibitors of Vif-APOBEC3G Binding. J Virol 81, 13235-13241(2007). Link
  5. Najima, Y. et al. High Mobility Group Protein-B1 Interacts with Sterol Regulatory Element-binding Proteins to Enhance Their DNA Binding. J Biol Chem 280, 27523-27532 (2005). Link
  6. Nakamura, K. et al. Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Measurement of Phox/Bem1p (PB1) Domain Heterodimerization. J Biomol Screen 13, 396-405 (2008). Link
  7. Porter, J. et al. Tetrahydroisoquinoline Amide Substituted Phenyl Pyrazoles as Selective Bcl-2 Inhibitors. Bioorg Med Chem Lett 19, 230-233 (2009). Link
  8. Zhou, V. et al. A Time-Resolved Fluorescence Resonance Energy Transfer-Based HTS Assay and a Surface Plasmon Resonance-Based Binding Assay for Heat Shock Protein 90 Inhibitors. Anal Biochem 331, 349-357 (2004). Link


PerkinElmer technical publications

  1. Hurskainen, P., Frang, H., Vikstrom, S., Karvinen, J. & Hemmilä, I. Heterogeneous and Homogeneous Time-resolved Fluorescence-based Assays for a Low-Affinity Binding Reaction. PerkinElmer Poster. Link


LANCE nuclear receptor assays


Journal articles

  1. Glickman, J.F. et al. A Comparison of ALPHAScreen, TR-FRET, and TRF as assay methods for FXR nuclear receptors. J Biomol Screen 7, 3-10 (2002). Link
  2. Dietz, M. et al. Comparative molecular profiling of the PPARα/γ activator aleglitazar: PPAR selectivity, activity and interaction with cofactors. ChemMedChem 7, 1101–1111 (2012).
  3. Gunther, J.R. et al. A Set of Time-Resolved Fluorescence Resonance Energy Transfer Assays for the Discovery of Inhibitors of Estrogen Receptor-Coactivator Binding. J Biomol Screen 14, 181-193 (2009). Link
  4. Kane, C.D. et al. Molecular Characterization of Novel and Selective Peroxisome Proliferator-Activated Receptor α Agonists with Robust Hypolipidemic Activity in Vivo. Mol Pharmacol 75, 296-306 (2009). Link
  5. Liu, J. et al. A Homogeneous in Vitro Functional Assay for Estrogen Receptors: Coactivator Recruitment. Mol Endocrinol 17, 346-355 (2003). Link
  6. Urizar, N.L. et al. A Natural Product that Lowers Cholesterol as an Antagonist Ligand for FXR. Science 296, 1703-1706 (2002). Link
  7. Wu, X., Sills, M.A. & Zhang, J. Further Comparison of Primary Hit Identification by Different Assay Technologies and Effects of Assay Measurement Variability. J Biomol Screen 10, 581-589 (2005). Link


LANCE kinase assays


Journal articles

  1. Zhu, W. et al. Synthesis and Biological Evaluation of Novel 6-Hydrazinyl-2,4-bismorpholino pyrimidine and 1,3,5-Triazine Derivatives as Potential Antitumor Agents. Archiv der Pharmazie (2012).
  2. McGhie, T. K., Martin, H. & Lunken, R. C. M. The combination of analytical-scale HPLC separation with a TR-FRET assay to investigate JAK2 inhibitory compounds in a Boysenberry drink. Food Funct (2012).
  3. Chen, S.-M. et al. Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. Biochemical Pharmacology (2012).Doti, N., Marasco, D., Pedone, C., Sabatella, M. & Ruvo, M. Optimizing a Kinase Assay for IKKB on an HTS Station. J Biomol Screen (2009). Link
  4. Jin, G. et al. Development and Comparison of Nonradioactive in Vitro Kinase Assays for NIMA-Related Kinase 2. Anal Biochem 358, 59-69 (2006). Link
  5. Moshinsky, D.J., Ruslim, L., Blake, R.A. & Tang, F. A Widely Applicable, High-Throughput TR-FRET Assay for the Measurement of Kinase Autophosphorylation: VEGFR-2 as a Prototype. J Biomol Screen 8, 447-452 (2003). Link
  6. Patnaik, D. et al. Identification of Small Molecule Inhibitors of the Mitotic Kinase Haspin by High-Throughput Screening Using a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay. J Biomol Screen 13, 1025-1034 (2008). Link
  7. Sabbatini, P. et al. Antitumor Activity of GSK1904529A, a Small-Molecule Inhibitor of the Insulin-like Growth Factor-I Receptor Tyrosine Kinase. Clin Cancer Res 15, 3058-3067 (2009). Link
  8. Zhang, W., Shor, B. & Yu, K. Identification and Characterization of a Constitutively T-Loop Phosphorylated and Active Recombinant S6K1: Expression, Purification, and Enzymatic Studies in a High Capacity Non-Radioactive TR-FRET Lance Assay. Protein Expres Purif 46, 414-420 (2006). Link


PerkinElmer technical publications

  1. Bielefeld-Sévigny, M. et al. Homogeneous and non-radioactive Cellular Assay Platforms for the Characterization of Kinase Inhibitors. Poster by PerkinElmer and University of Lausanne. Link
  2. Blouin, J. et al. Novel Substrates for Fluorescence-based Protein Tyrosine Kinase Assays. PerkinElmer Poster. Link
  3. Caron, M. et al. Development and Optimization of Kinase Assays using New LANCE Ultra TR-FRET Reagents. PerkinElmer Application Note. Link
  4. Legault, M., Roby, P., Beaudet, L. & Rouleau, N. Comparison of LANCE Ultra TR-FRET to PerkinElmer's Classical LANCE TR-FRET Platform for Kinase Applications. PerkinElmer Application Note. Link
  5. Legault, M. et al. Comparison of LANCE Ultra with Other TR-FRET Platforms for a Src Tyrosine Kinase Assay. PerkinElmer Poster. Link
  6. Padros, J. et al. Development, Automation, and Miniaturization of High-Throughput Serine/Threonine Kinase Assays Using the LANCE Ultra Platform. PerkinElmer Poster. Link
  7. True, D., Kasila, P. & Harney, H. Comparison of Kinase Assay Technologies for High Throughput Screening. PerkinElmer Poster (2002). Link
  8. Warner, G. Measurement of p38/MAPK Activity Using LANCE. PerkinElmer Application Note. Link
  9. Miniaturization of LANCE Kinase Assays. PerkinElmer Application Note. Link
  10. How to Optimize a Tyrosine Kinase Assay Using Time Resolved Fluorescence-Based LANCE Detection. PerkinElmer Application Note. Link


Receptor-ligand assays


Journal articles

  1. Gasser, J., Hehl, M. & Millward, T.A. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Phosphatidylserine Exposure on Apoptotic Cells. Anal Biochem 384, 49-55 (2009). Link
  2. Moore et al. A Homogenous 384-Well High Throughput Screen for Novel Tumor Necrosis Factor Receptor: Ligand Interactions Using Time Resolved Energy Transfer. J Biomol Screen 4, 205-214 (1999). Link
  3. Pandit, S. et al. Functional Analysis of Sites within PCSK9 Responsible for Hypercholesterolemia. J Lipid Res 49, 1333-1343 (2008). Link
  4. Stenroos, K. et al. Homogeneous Time-Resolved IL-2-IL-2R Alpha Assay using Fluorescence Resonance Energy Transfer. Cytokine 10, 495-499 (1998). Link
  5. Yang, X. et al. Binding Characterization of the Interleukin-13 Signaling Complex and Development of a Ternary Time-Resolved Fluorescence Resonance Energy Transfer Assay. Anal Biochem 376, 206-212 (2008). Link


PerkinElmer technical publications

  1. Frang, H., Lahteenmaki, K., Hurskainen, P. & Hemmilä, I. Homogeneous Receptor Ligand Binding Assay Based on TR-FRET. PerkinElmer Application Note. Link
  2. Stenroos, K. et al. Homogeneous Time-Resolved Fluorescence Energy Transfer Assay (LANCE) for the Determination of IL-2/IL-2 Receptor alpha interaction. Poster by Abo Akademi University and PerkinElmer. Link


Receptor dimerization assays


Journal articles

  1. Carrillo, J.J., Pediani, J. & Milligan, G. Dimers of Class A G Protein-Coupled Receptors Function via Agonist-Mediated Trans-Activation of Associated G Proteins. J Biol Chem 278, 42578-42587 (2003). Link
  2. Kong, M.M.C., Fan, T., Varghese, G., O'dowd, B.F. & George, S.R. Agonist-Induced Cell Surface Trafficking of an Intracellularly Sequestered D1 Dopamine Receptor Homo-Oligomer. Mol Pharmacol 70, 78-89 (2006). Link
  3. McVey, M. et al. Monitoring Receptor Oligomerization Using Time-Resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer. The Human Delta-Opioid Receptor Displays Constitutive Oligomerization at the Cell Surface, which is not Regulated by Receptor Occupancy. J. Biol. Chem 276, 14092-14099 (2001). Link
  4. So, C.H. et al. Calcium Signaling by Dopamine D5 Receptor and D5-D2 Receptor Hetero-Oligomers Occurs by a Mechanism Distinct from That for Dopamine D1-D2 Receptor Hetero-Oligomers. Mol Pharmacol 75, 843-854 (2009). Link


Cell-based assays


Journal articles

  1. Gasser, J., Hehl, M. & Millward, T.A. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Phosphatidylserine Exposure on Apoptotic Cells. Anal Biochem 384, 49-55 (2009). Link
  2. Hassan, N. et al. Development of an Insect Cell-Based Assay for Detection of Kinase Inhibition using NF-kappaB Inducing Kinase as a Paradigm. Biochem J 419, 65-73 (2008). Link
  3. Lundin, K., Blomberg, K., Nordström, T. & Lindqvist, C. Development of a Time-Resolved Fluorescence Resonance Energy Transfer Assay (Cell TR-FRET) for Protein Detection on Intact Cells. Anal Biochem 299, 92-97 (2001). Link
  4. McVey, M. et al. Monitoring Receptor Oligomerization Using Time-Resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer. The Human Delta-Opioid Receptor Displays Constitutive Oligomerization at the Cell Surface, Which is Not Regulated by Receptor Occupancy. J. Biol. Chem 276, 14092-14099 (2001). Link
  5. Stenroos, K. et al. Homogeneous Time-Resolved IL-2-IL-2R Alpha Assay Using Fluorescence Resonance Energy Transfer. Cytokine 10, 495-499 (1998). Link


PerkinElmer technical publications

  1. Bielefeld-Sévigny, M. et al. Homogeneous and non-radioactive Cellular Assay Platforms for the Characterization of Kinase Inhibitors. Poster by PerkinElmer and University of Lausanne. Link


Immunoassays/analyte detection


Journal articles

  1. Blomberg, K., Hurskainen, P. & Hemmilä, I. Terbium and Rhodamine as Labels in a Homogeneous Time-Resolved Fluorometric Energy Transfer Assay of the Beta Subunit of Human Chorionic Gonadotropin in Serum. Clin. Chem 45, 855-861 (1999). Link
  2. Kuroda, K. et al. Efficient Antibody Production upon Suppression of O Mannosylation in the Yeast Ogataea minuta. Appl. Environ. Microbiol. 74, 446-453 (2008). Link
  3. Qin, Q., Peltola, O. & Pettersson, K. Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin. Clin Chem 49, 1105-1113 (2003). Link
  4. Yu, V. et al. High capacity Homogeneous Non-Radioactive Cortisol Detection Assays for Human 11Beta-Hydroxysteroid Dehydrogenase Type 1. Assay Drug Dev Technol 5, 105-115 (2007). Link


PerkinElmer technical publications

  1. Beaudet, L. et al. New LANCE Assays for the High Throughput Quantitation of Cytokine Biomarkers. PerkinElmer Poster. Link


Ubiquitination


Journal articles

  • Boisclair, M.D. et al. Development of a Ubiquitin Transfer Assay for High Throughput Screening by Fluorescence Resonance Energy Transfer. J Biomol Screen 5, 319-328 (2000). Link
  • Hong, C.A. et al. Development of a High Throughput Time-Resolved Fluorescence Resonance Energy Transfer Assay for TRAF6 Ubiquitin Polymerization. Assay Drug Dev Technol 1, 175-180 (2003). Link
  • Murray, M.F. et al. A High-Throughput Screen Measuring Ubiquitination of p53 by Human mdm2. J Biomol Screen 12, 1050-1058 (2007). Link