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The LANCE® indirect assay uses an unlabeled anti-phospho antibody with a Europium-labeled secondary antibody. This allows you to use your own unlabeled anti-phospho antibody with one of our catalog Eu-labeled secondary antibodies.
Bader, B. et al. A cGMP-Dependent Protein Kinase Assay for High Throughput Screening Based on Time-Resolved Fluorescence Resonance Energy Transfer. J Biomol Screen 6, 255-264 (2001). Link
Zhang, W., Shor, B. & Yu, K. Identification and Characterization of a Constitutively T-Loop Phosphorylated and Active Recombinant S6K1: Expression, Purification, and Enzymatic Studies in a High Capacity Non-Radioactive TR-FRET Lance assay. Protein Expres Purif 46, 414-420 (2006). Link
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