• Overview
• Products and catalog numbers
• Protocols
• FAQs

Overview

PerkinElmer offers two luciferase substrates for in vitro, in vivo and ex vivo bioluminescent imaging: D-luciferin and coelenterazine H. D-luciferin is a substrate for Firefly luciferase (such as Red-FLuc), and is used in conjunction with our Bioware Ultra bioluminescent oncology cell lines or cells transfected with our RediFect™ Red-FLuc and Red-FLuc-GFP lentiviral particles for in vitro, in vivo or ex vivo imaging. Coelenterazine H is a substrate for Renilla and Gaussia luciferase, and can be used for in vivo imaging with cells transfected with our RediFect Green RenLuc lentiviral particles, or when either of these luciferases is used as a reporter. The respective substrate is necessary for bioluminescent visualization in vitro, in vivo or ex vivo.

After the bioluminescent cell line expressing firefly luciferase, Renilla luciferase, or Gaussia luciferase is established in a mouse or other small animal model, the luciferin or coelenterazine substrate is injected. The substrate is then used by the bioluminescent reporter to produce light within the cell line, which allows visualization of the bioluminescent cells within the mouse (or other) model. This luminescence can be visualized and measured using an appropriate 2D or 3D imager.

Products and catalog numbers

*Pre-formulated with a rapidly clearing fluorescent dye to validate the integrity of your substrate injection, so with a quick fluorescent image on your imaging system you know immediately if an injection failed, and you can normalize your bioluminescence results

**RediJect products are supplied in a ready-to-use injectable format

Protocols

• Preparation of luciferin for in vitro or in vivo assays (can be used for Cat. No. 122796)
  
• Determining luciferin kinetic curve for your model 
• Using Coelenterazine H for in vivo imaging

FAQs