Bioluminescent Cell Lines

• Overview
• Product selection tables and culturing information
  • Blood cancer cell lines
  • Brain oncology cell lines
  • Breast cancer cell lines
  • Colorectal oncology cell lines
  • Fibrosarcoma cell lines
  • Liver oncology cell lines
  • Lung cancer cell lines
  • Melanoma cell lines
  • Ovarian cancer cell lines
  • Pancreatic cancer cell lines
  • Prostate cancer cell lines
  • Lentiviral particles for creating your own bioluminescent cells
• Substrates
• Protocols
• Citations

Overview

Our bioluminescent oncology cell lines (also known as Bioware® Brite cell lines) are stably transfected with the firefly luciferase reporter gene from Luciola Italica (Red-Fluc) that allows you to visualize the growth of the cells in vivo. Once the cell lines are injected into an appropriate mouse mode,subsequent expression of the optimized Red-Fluc luciferase gene allows you to to monitor early tumor development, monitor tumor growth and metastases even in deep tissue, quantify tumor burden in a whole animal model, and follow therapeutic responses non-invasively. PerkinElmer currently offers oncology cell lines covering a range of cancer models including breast, colorectal, prostate, lung, and lymphoma.

• Bioware Brite cell lines contain the brighter, red-shifted Red- Fluc reporter gene and are bright enough to measure tumor growth in deep tissues, well before the tumors are palpable, allowing you to start collecting data at time points and tissue depths not possible with traditional micrometer measurements.
• Bioware Brite Ultra Green are dual-labeled cell lines expressing Red-Fluc luciferase and GFP green fluorescent protein (allowing you to monitor tumor growth in vivo with luciferase and ex vivo (in analyses such as immunohistochemistry) with the fluorescent protein. PerkinElmer currently offers dual labeled cell lines in breast and prostate cancer models.

 
Figure 1: 1 million HT1080-Red-FLuc cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Tumor growth was monitored for luciferase expression using the PerkinElmer IVIS® Spectrum at various time points. Mice were imaged 10 minutes post i.p. injection of luciferin at 150mg/kg at various time points. The image above shows tumor growth from a representative mouse.

All our cell lines meet the highest standards and are confirmed pathogen-free. The in vitro growth rates of these cell lines are similar to their parental lines and the stability of reporter expression is tested for a minimum of four weeks.

We also provide a wide range of bioluminescent pathogenic bacteria (both Gram-positive and Gram-negative) that can be used for drug discovery both in vitro and in vivo (including biofilm models) when used with our imaging systems. These light-producing microorganisms have been shown to work in a variety of acute and chronic infection models including pneumonia, meningitis, sepsis and biofilm infections.

Product selection table and culturing information

Blood cancer cell lines

Brain oncology

Breast cancer cell lines

Colorectal oncology

Fibrosarcoma

Liver oncology

Lung cancer

Melanoma

Ovarian cancer

Pancreatic cancer

Prostate cancer

Lentivirus particles for creating your own bioluminescent cells

View our lentiviral particles page for products and information on creating your own bioluminescent cells.

Substrates

Please see our page on D-luciferin substrates.

Protocols

• Culture Guidelines
• Cell preparation and imaging procedure – subcutaneous 
• Cell preparation and imaging procedure – intravenous 
• Cell preparation and imaging procedure – ex vivo
• Examples of validated models and cell injection number
  
• Imaging Procedure
• Preparation of luciferin for in vitro or in vivo assays using Cat. No. 122796
• RediJect D-luciferin protocol
• RediJect D-luciferin Ultra protocol

Citations

Browse or search our bioluminescent oncology cell line citations.