AlphaScreen SureFire Instrument Options and Settings
Alpha technology requires excitation at 680 nm, reading emission at 520-620 nm (AlphaScreen) or 615 nm (AlphaLISA). A laser excitation source (rather than a Xenon Flashlamp) is usually needed, as an intense light source is required to generate signal. Many basic fluorescence plate readers are not equipped to run Alpha assays. We recommend using our EnVision® (with Alpha module), Fusion®, or EnSpire™ Multilabel Plate Reader, each of which comes with pre-set Alpha settings (view our instrument product pages). You can use the same generic instrument protocol for AlphaScreen and AlphaLISA assays. If you are using a plate reader from another company, we recommend that you check to see if the manufacturer has validated the instrument for an AlphaScreen or AlphaLISA assay. This information is usually found on the company's website. PerkinElmer has not validated other instruments, and we would recommend that you speak to the instrument manufacturer for information on the recommended settings to use.
If you are using an EnVision Multilabel Plate Reader, you may notice there are two instrument protocols for Alpha. One is the HTS Alpha protocol, and other is the standard Alpha protocol. The HTS Alpha protocol is to be used when you are performing high-throughput assays, and when the time it takes to read each plate is an important factor for you.