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SDS and bead interference

The table below shows results from a compound interference study. We tested a panel of 24 compounds in a QC assay using our AlphaLISA® toolbox bead products. The information below can be used for guidance as to whether SDS might interfere in assays involving particular bead products, and at what SDS concentration interference might occur. These data are derived from single experiments and should serve more as a guide rather than a precise value, as it is unlikely you will be using the exact same assay design as used to generate the data below. Interference concentrations may vary depending on the assay components. It is possible your assay will tolerate higher or lower concentration than what is shown.

Tested up to 3%
Catalog NumberProductNo effect (10%)50% loss% of inhibition at max conc
AL125Streptavidin AlphaLISA Acceptor Beads0.070.196
AL126Protein L AlphaLISA Acceptor Beads0.070.08100
AL127Anti-FITC AlphaLISA Acceptor Beads0.070.09100
AL128 (biotin)Anti-6xHis AlphaLISA Acceptor Beads0.080.09100
AL128 (GSH)Anti-6XHis AlphaLISA Acceptor Beads0.0010.002100
AL129Anti-V5 AlphaLISA Acceptor Beads0.030.05100
AL130Anti-mouse IgM AlphaLISA Acceptor Beads0.010.02100
AL131Anti-chicken IgY AlphaLISA Acceptor Beads0.080.09100
AL132Anti-sheep IgG AlphaLISA Acceptor Beads0.090.198
AL133Anti-GFP AlphaLISA Acceptor Beads0.030.07100
AL134Anti-MBP AlphaLISA Acceptor Beads0.0060.008100
AL136Strep-Tactin AlphaLISA Acceptor Beads0.060.196
AS102Protein A Alpha Donor Beads0.030.04100
AS103Anti-FLAG Alpha Donor Beads0.080.1100
AS104Anti-mouse IgG Alpha Donor Beads0.030.0599
AS105Anti-rabbit IgG Alpha Donor Beads0.070.196
AS106Strep-Tactin Alpha Donor Beads0.060.06100
AL900AlphaLISA TruHits kit 2 ug/mL0.1>333
AL900AlphaLISA TruHits kit 20 ug/mL0.089>332

Refer to the figure below for more detail as to what the values in the table above indicate. Please note that the figure is provided as a general example to explain how the values in our interference tables were determined. The table above indicates that an assay testing the effect of SDS using anti-6X His AlphaLISA Acceptor beads (with glutathione Donor beads) showed a half-maximal inhibition of signal when SDS was used at a final concentration of 0.002%. When 3% SDS was included in the assay, 100% signal inhibition was observed.

Figure: Key for how the values in our interference tables are derived. The “no effect” concentration was obtained by extrapolating the MAX - 10% MAX counts. Refer to the table above for actual interference values for each toolbox bead product.