Cookies on PerkinElmer
PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. This may include cookies from third party websites. If you continue without changing your settings, we will assume that you consent to receive cookies from this website. You can change your cookie settings at any time. To learn more, please review our cookie policy, which includes information on how to manage your cookies.

AlphaLISA 5 μL vs. 20 μL Sample Volume

  • Increasing the volume of the sample while keeping the overall reaction volume can increase the sensitivity of the assay. This is because you are adding more molecules of analyte into the well.
  • Please note that increasing the volume of sample in a 50 µL reaction might also increase any potential assay interference arising from your sample or matrix. We do not recommend adjusting the volume of your sample if you are using serum samples.
5 uL vs 20 uL sample