PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. This may include cookies from third party websites. If you continue without changing your settings, we will assume that you consent to receive cookies from this website. You can change your cookie settings at any time. To learn more, please review our cookie policy, which includes information on how to manage your cookies.

Gelatin and bead interference

The table below shows results from a compound interference study. We tested a panel of 24 compounds in a QC assay using our AlphaLISA® toolbox bead products. The information below can be used for guidance as to whether gelatin might interfere in assays involving particular bead products, and at what gelatin concentration interference might occur. These data are derived from single experiments and should serve more as a guide rather than a precise value, as it is unlikely you will be using the exact same assay design as used to generate the data below. Interference concentrations may vary depending on the assay components. It is possible your assay will tolerate higher or lower concentration than what is shown.

Tested up to 1%
Catalog NumberProductNo effect (10%)50% loss% of inhibition at max conc
AL125Streptavidin AlphaLISA Acceptor Beads>1>10
AL126Protein L AlphaLISA Acceptor Beads>0.5>117
AL127Anti-FITC AlphaLISA Acceptor Beads>1>10
AL128 (biotin)Anti-6xHis AlphaLISA Acceptor Beads0.3>132
AL128 (GSH)Anti-6XHis AlphaLISA Acceptor Beads0.81.050
AL129Anti-V5 AlphaLISA Acceptor Beads>0.1>0.10
AL130Anti-mouse IgM AlphaLISA Acceptor Beads>1>10
AL131Anti-chicken IgY AlphaLISA Acceptor Beads0.21.052
AL132Anti-sheep IgG AlphaLISA Acceptor Beads>0.1>0.10
AL133Anti-GFP AlphaLISA Acceptor Beads>1>10
AL134Anti-MBP AlphaLISA Acceptor Beads>1>10
AL136Strep-Tactin AlphaLISA Acceptor Beads>0.1>0.10
AS102Protein A Alpha Donor Beads>1>10
AS103Anti-FLAG Alpha Donor Beads>1>10
AS104Anti-mouse IgG Alpha Donor Beads>1>10
AS105Anti-rabbit IgG Alpha Donor Beads>1>10
AS106Strep-Tactin Alpha Donor Beads>1>10
AL900AlphaLISA TruHits kit 2 ug/mL>1>10
AL900AlphaLISA TruHits kit 20 ug/mL>0.1>0.14

Refer to the figure below for more detail as to what the values in the table above indicate. Please note that the figure is provided as a general example to explain how the values in our interference tables were determined. The table above indicates that an assay testing the effect of gelatin using anti-6X His AlphaLISA Acceptor beads (with glutathione Donor beads) showed little effect on the assay signal at final gelatin concentrations up to ~0.8%. Gelatin did show a half-maximal inhibition of signal when used at a final concentration of 1.0%.

Figure: Key for how the values in our interference tables are derived. The “no effect” concentration was obtained by extrapolating the MAX - 10% MAX counts. Refer to the table above for actual interference values for each toolbox bead product.