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DMSO and bead interference

The table below shows results from a compound interference study. We tested a panel of 24 compounds in a QC assay using our AlphaLISA® toolbox bead products. The information below can be used for guidance as to whether DMSO might interfere in assays involving particular bead products, and at what DMSO concentration interference might occur. These data are derived from single experiments and should serve more as a guide rather than a precise value, as it is unlikely you will be using the exact same assay design as used to generate the data below. Interference concentrations may vary depending on the assay components. It is possible your assay will tolerate higher or lower concentration than what is shown.



Tested up to 10%
Catalog NumberProductNo effect (10%)50% loss% of inhibition at max conc
AL125Streptavidin AlphaLISA Acceptor Beads>0.5>1.887
AL126Protein L AlphaLISA Acceptor Beads1.49.751
AL127Anti-FITC AlphaLISA Acceptor Beads2.09.551
AL128 (biotin)Anti-6xHis AlphaLISA Acceptor Beads1.02.986
AL128 (GSH)Anti-6XHis AlphaLISA Acceptor Beads3.0>1047
AL129Anti-V5 AlphaLISA Acceptor Beads1.02.193
AL130Anti-mouse IgM AlphaLISA Acceptor Beads0.21.491
AL131Anti-chicken IgY AlphaLISA Acceptor Beads0.0030.686
AL132Anti-sheep IgG AlphaLISA Acceptor Beads0.82.380
AL133Anti-GFP AlphaLISA Acceptor Beads0.040.697
AL134Anti-MBP AlphaLISA Acceptor Beads1.01.986
AL136Strep-Tactin AlphaLISA Acceptor Beads7.6>1038
AS102Protein A Alpha Donor Beads0.41.591
AS103Anti-FLAG Alpha Donor Beads1.64.662
AS104Anti-mouse IgG Alpha Donor Beads0.21.190
AS105Anti-rabbit IgG Alpha Donor Beads0.51.887
AS106Strep-Tactin Alpha Donor Beads1.0>1022
AL900AlphaLISA TruHits kit 2 ug/mL2.53.080
AL900AlphaLISA TruHits kit 20 ug/mL0.14.073

Refer to the figure below for more detail as to what the values in the table above indicate. Please note that the figure is provided as a general example to explain how the values in our interference tables were determined. The table above indicates that an assay testing the effect of DMSO using anti-6X His AlphaLISA Acceptor beads (with glutathione Donor beads) showed little effect on the assay signal at final DMSO concentrations up to ~3%. When 10% DMSO was included in the assay, 47% signal inhibition was observed.

interferencetesting16_ASK.jpg
Figure: Key for how the values in our interference tables are derived. The “no effect” concentration was obtained by extrapolating the MAX - 10% MAX counts. Refer to the table above for actual interference values for each toolbox bead product.