Western Blotting

Blotting is one of the most prevalent and useful analytical methods for the detection and quantification of proteins and nucleic acids. With PerkinElmer’s range of reagents for detecting proteins and nucleic acids in blotting applications, you can have the highest level of sensitivity and specificity for confidence in your results.

Researchers have depended on western blotting for decades to perform proteomic studies and validate many experimental results, including post translational and CRISPR studies. With chemiluminescent, fluorescent, and colorimetric detection methods, PerkinElmer’s enhanced western blotting substrates, in addition to our nucleic acid detection reagents, can keep your research studies on track and moving forward.

Chemiluminescent Blotting Substrates

A trusted portfolio of accurate and efficient horseradish peroxidase (HRP) or alkaline phosphatase (AP) blotting agents for Western or Southern blotting applications.

  • Western Lightning ONE – Premixed one-component chemiluminescent HRP substrate offering a range of protein sensitivity from mid picogram to low femtogram for more consistent results.​
  • Western Lightning – Two-component chemiluminescent HRP substrate for reliable and sensitive protein detection.

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Chromogenic Substrates

For the convenience of direct colorimetric visualization of results without the need for a film or imaging instrument, count on our DAB and BCIP/NBT substrates for use in chromogenic assays

  • DAB to detect horseradish peroxidase (HRP)
  • BCIP/NBT to detect alkaline phosphatase (AP)

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Secondary Antibodies & Proteins

We offer a variety of HRP, AP, or Biotin labeled affinity-purified secondary antibodies and proteins offering excellent selectivity for blotting, as well as for ELISA, histochemical, and cytochemical staining procedures.

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Transfer membranes

Ensure accurate detection with high performance transfer membranes for blotting and other applications.

  • Protran - Nitrocellulose membranes for high protein binding capacity and retention of small proteins in chromogenic and chemiluminescent Western blotting.
  • GeneScreen - Neutral nylon membranes for nucleic acid binding in chemiluminescent and chromogenic Northern and Southern blotting.
  • GeneScreen Plus – Positively charged nylon membranes for radiometric assays.

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